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Inhibition of cell wall synthesis by penicillin resulted in disruption of the cell wall and lysis of the cell.
One class of dosage suppressors is composed of genes encoding cell wall proteins, indicating that alterations in cell wall structure can rescue the cell lysis in ram cells.
Presumably, the shock treatments that elicit bursting disrupt the balance of wall synthesis and lysis responsible for cell wall extension.
Samples for alkaline elution were kept in ice during irradiation and successive treatments until the lysis step.
However, apart from using an unstable enzyme, our first route had other problems, caused by cell lysis (rupture of the cell walls) during the biotransformation, which hampered product recovery.
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